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KMID : 0378019670100100073
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New Medical Journal
1967 Volume.10 No. 10 p.73 ~ p.81
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Aging Changes in Tissue Mast Cells in White Rats
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Abstract
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The tissus mast cells were described in literature as a sorce of heparin, histamine, hyaluronic acid and serotonin. Although the cells in the connective tissue of the body were studied carefully, the correlation between the frequency of their appearance and ages of animals had not done satisfactorily determined. The author was interested in this subject and examined the mast cells in the mesenteries, skin, pancreas, uterus, and ovaries of rats aged 1, 3, 6, 12, 18 and 24 months. Each of the age groups consisted of five rats.
Specimens were taken from the organs under a fresh condition(except specimens from mesenteries)and prepared by a general method for tissue mast cells. The mesenteries, thin membranes were cut from the animals and made into film preparations.
In observation, the author divided the tissue mast cells of rats into four- types based on the distribution of the specific granulestype A,B and C which corespond to the cell granulated clossly, loosely or rarely in the cytoplasm and type D indicated the granules scattered outside of the cell body respectively.
For the purpose of comparing the numbers of mast cells in individual organs or different age groups the author counted the number of cells by the magnification of 450 X. The average number of cells which was considered the relative number of each: group, was comFesed based upon the total number in 250 microscopic fields from each age group.
The results were as follows:
1. The mast cells in the examined rats of different ages were most highly developed in the mesentery and the number of mast cells in each organ, was found in a descending order: skin, uterus, overy and pancreas.
2. The relative numbers of mast cells in the mesentery, pancreas and uterus increased gradually with age and showed the highest level at about the 18th month in the former two organs and at the 24th month in the Iatter.
3. Those of skin and ovary showed already the maximal value at about the third month in the former and at the sixth month in the latter, showed a constant number of cells in older age.
4. In general, the mast cells found in the mesenteries were mostly spherical or ovoid in shape but those in the other organs showed fairly many deformities. ~
5. The granules of the tissue :east cells of rats were in general packed clossly in the cytoplasm and rarely found outside the cell body. Its stainability was intensely basophile- and metachromatic to usual basic aniline dyes regardless of the age of the animal
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